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We analyzed dendritic spine parameters in hippocampal and cortical brain regions after targeted expression of EGFP to allow high-resolution imaging, followed by algorithm-based evaluation of mice of both sexes from adolescence to old age. We observed an age-dependent loss of thin spines under most conditions as an indicator of a loss of synaptic plasticity in older mice. We also observed an increase in the protein levels of PSD and Arc in the hippocampus and cortex, respectively. Our data also indicated that spine morphology differs between sexes in a region-specific manner.
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I was coming home from a womens conference late one night from green bay when this trucker pulled along side me and honked his horn. I thought nothing of it at first, then this wicked thought came over me to put the inside light on of my minivan and pull my skirt up a little to see if he actually could see me. He not only saw me but blew his air horn several times. I was so excited that I pulled my skirt up all the way revealing my red see thru panties and also unbuttoned my blk blouse to reveal my matching bra and this got the trucker very excited. I then saw a sign for a rest stop about 2 miles up the road and pulled ahead of the trucker and pulled off into the exit and he followed.
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